| The World Cocoa Foundation (formerly the American Cocoa Research Institute has had long relationship with the CRU, starting
in the 1960's with their invaluable support of W.S.
Chalmers' collection expeditions. More recently
(from 1993-1998), an evaluation of cocoa butterfat
content of 450 accessions of the ICG,
T was undertaken with funding from the WCF when it was known as ACRI.
Details of the current research project financed
by WCF are summarised here. CRU staff members involved
in the project are also listed and they may be contacted
directly with any questions or comments.
Evaluation
of cacao germplasm for resistance to Witches' Broom
disease
Witches'
Broom (WB) disease, caused by Crinipellis perniciosa,
is one of the major diseases faced by cocoa producers
in Latin America. The disease is endemic to the
forests of the Amazon basin and it has long been
known in the Upper Amazon regions of Brazil, with
pod losses in this region reaching 95% on occasions.
Witches' Broom disease was first observed in Trinidad
in 1927 and the search for resistance to Witches'
Broom disease began in the 1930s at the Imperial
College of Tropical Agriculture (now the University
of the West Indies). Since that time, research into
cacao and cacao diseases in Trinidad has continued
uninterrupted to the present day. In the 1980's,
various collections of cacao germplasm previously
brought into Trinidad were re-established on a single
site to form the International Cocoa Genebank, Trinidad
(ICG, T). Almost half of these accessions had been
selected for the lack of of WB symptoms on the parent
tree in the area where they were found.
Detailed information is required on the level of
resistance to WB in accessions at the ICG, T, with
a view to selecting parents for germplasm enhancement.
In order to acquire this information, a project
for the mass screening of cacao germplasm is in
progress.
Phase I of this project took place from July, 1998
to July, 2003. Phase II began in August, 2003 A
spray inoculation method is being used, based on
an automated belt spray system developed by Purdy
et al.., (1997), which was designed for inoculation
of large numbers of seedlings. We have adapted the
system to screen grafted clones rather than seedlings.
The plant material to be screened is collected from
the ICG,T as budwood and top-grafted onto TSH rootstocks,
and then kept for three months to allow the grafts
to become established.
During Phase I of the project we screened over 700
accessions from the ICG,T.
Project
Staff
Penina Deberdt (until March, 2008) Annelle Holder-John
Romina Umaharan
Surendra S.-Maharaj (until June, 2008)
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